Gutezimbere uburyo butaziguye bwa ELISA kugirango hamenyekane syndrome ya porcine acute diarrhea coronavirus IgG antibody ishingiye kuri proteine ​​recombinant spike

Indwara yitwa Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) ni porcine enteric pathogenic coronavirus iherutse kuvumburwa ishobora gutera impiswi y'amazi mu ngurube zikivuka kandi bigatera igihombo gikomeye mu bukungu ku ngurube.Kugeza ubu, nta buryo bukwiye bwa serologiya bwo gusuzuma imikorere y’indwara ya SADS-CoV n’urukingo, bityo rero hakenewe byihutirwa gukoresha imiti ifatika ifitanye isano na immunosorbent (ELISA) kugira ngo ikemure iki kibazo.Hano, plasmid ya recombinant yerekana proteine ​​ya SADS-CoV spike (S) yahujwe na domeni ya IgG Fc yubatswe kugirango ikore baculovirus recombinant kandi igaragarira muri selile HEK 293F.Poroteyine S-Fc isukurwa hamwe na protein G resin kandi ikagumana reaction hamwe na antibodiyite zirwanya abantu Fc na anti-SADS-CoV.Noneho poroteyine S-Fc yakoreshejwe mugutezimbere ELISA itaziguye (S-iELISA) no kunoza imiterere ya S-iELISA.Kubera iyo mpamvu, mu gusesengura agaciro ka OD450nm ya 40 SADS-CoV mbi ya sera mbi yemejwe na immunofluorescence assay (IFA) hamwe n’iburengerazuba bw’iburengerazuba, agaciro kaciwe kiyemeje kuba 0.3711.Coefficient yo gutandukana (CV) ya sera 6 nziza ya SADS-CoV imbere no hagati ya S-iELISA byose byari munsi ya 10%.Ikizamini cya cross-reactivite cyerekanye ko S-iELISA idafite reaction-reaction hamwe nizindi virusi ya porcine sera.Byongeye kandi, hashingiwe ku gutahura ingero 111 z’amavuriro ya serumu, igipimo rusange cy’impanuka cya IFA na S-iELISA cyari 97.3%.Ikizamini cyo kutabuza virusi hamwe n’indangagaciro 7 zitandukanye za OD450nm za serumu zerekanye ko agaciro ka OD450nm kagaragajwe na S-iELISA kajyanye neza n’ikizamini cyo kutabuza virusi.Hanyuma, S-iELISA yakorewe kuri serumu 300 yingurube.Ibikoresho byubucuruzi byizindi porcine enterovirusi byerekanaga ko IgG igipimo cyiza cya SADS-CoV, TGEV, PDCoV na PEDV cyari 81.7%, 54%, na 65.3%., 6%.Ibisubizo byerekana ko S-iELISA yihariye, yunvikana, kandi yororoka, kandi irashobora gukoreshwa mugutahura ubwandu bwa SADS-CoV mubikorwa byingurube.Iyi ngingo irinzwe nuburenganzira.uburenganzira bwose burabitswe.